Abstract
Infective endocarditis affects approximately 100,000 individuals in the USA. Medical advances have contributed to the rise of the disease, and no new therapies have emerged in the last 50 years to control the surge of this life-threatening infection. The rabbit vascular physiology and immune response mechanisms are similar to humans. Hence, the rabbit model of infective endocarditis is an excellent research tool with which to address many questions regarding development of endocarditis, for the testing of new therapies, and for the study of the molecular mechanisms used by infectious agents to cause disease. This chapter describes the surgical procedure required to study infective endocarditis in damaged native valves, therefore closely mimicking human disease.
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References
Fowler VG, Durack DT (1994) Infective endocarditis. Curr Opin Cardiol 9:389–400
Durack DT, Lukes AS, Bright DK (1994) New criteria for diagnosis of infective endocarditis: utilization of specific echocardiographic findings. Duke Endocarditis Service. Am J Med 96:200–209
Cheung AL, Yeaman MR, Sullam PM, Witt MD, Bayer AS (1994) Role of the sar locus of Staphylococcus aureus in induction of endocarditis in rabbits. Infect Immun 62:1719–1725
van Wamel W, Xiong YQ, Bayer AS, Yeaman MR, Nast CC, Cheung AL (2002) Regulation of Staphylococcus aureus type 5 capsular polysaccharides by agr and sarA in vitro and in an experimental endocarditis model. Microb Pathog 33:73–79
Xiong YQ, Bayer AS, Yeaman MR, Van Wamel W, Manna AC, Cheung AL (2004) Impacts of sarA and agr in Staphylococcus aureus strain Newman on fibronectin-binding protein A gene expression and fibronectin adherence capacity in vitro and in experimental infective endocarditis. Infect Immun 72:1832–1836
Xiong YQ, Fowler VG, Yeaman MR, Perdreau-Remington F, Kreiswirth BN, Bayer AS (2009) Phenotypic and genotypic characteristics of persistent methicillin-resistant Staphylococcus aureus bacteremia in vitro and in an experimental endocarditis model. J Infect Dis 199:201–208
Chuang ON, Schlievert PM, Wells CL, Manias DA, Tripp TJ, Dunny GM (2009) Multiple functional domains of Enterococcus faecalis aggregation substance Asc10 contribute to endocarditis virulence. Infect Immun 77:539–548
Frank KL, Guiton PS, Barnes AM, Manias DA, Chuang-Smith ON, Kohler PL et al (2013) AhrC and Eep are biofilm infection-associated virulence factors in Enterococcus faecalis. Infect Immun 81:1696–1708
Schlievert PM, Gahr PJ, Assimacopoulos AP, Dinges MM, Stoehr JA, Harmala JW et al (1998) Aggregation and binding substances enhance pathogenicity in rabbit models of Enterococcus faecalis endocarditis. Infect Immun 66:218–223
Fan J, Zhang Y, Chuang-Smith ON, Frank KL, Guenther BD, Kern M et al (2012) Ecto-5'-nucleotidase: a candidate virulence factor in Streptococcus sanguinis experimental endocarditis. PLoS One 7:e38059
Nallapareddy SR, Singh KV, Murray BE (2008) Contribution of the collagen adhesin Acm to pathogenesis of Enterococcus faecium in experimental endocarditis. Infect Immun 76:4120–4128
Nallapareddy SR, Singh KV, Sillanpaa J, Garsin DA, Hook M, Erlandsen SL et al (2006) Endocarditis and biofilm-associated pili of Enterococcus faecalis. J Clin Invest 116:2799–2807
Hindes RG, Willey SH, Eliopoulos GM, Rice LB, Eliopoulos CT, Murray BE et al (1989) Treatment of experimental endocarditis caused by a beta-lactamase-producing strain of Enterococcus faecalis with high-level resistance to gentamicin. Antimicrob Agents Chemother 33:1019–1022
Schlievert PM, Dunny GM, Stoehr JA, Assimacopoulos AP (1997) Aggregation and binding substances enhance pathogenicity in a rabbit model of Enterococcus faecalis endocarditis. Adv Exp Med Biol 418:789–791
Acknowledgment
This work was supported by the University of Iowa Carver College of Medicine Startup to WSP and PMS. We thank Katarina Kulhankova for the pictures.
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Salgado-Pabón, W., Schlievert, P.M. (2016). Aortic Valve Damage for the Study of Left-Sided, Native Valve Infective Endocarditis in Rabbits. In: Brosnahan, A. (eds) Superantigens. Methods in Molecular Biology, vol 1396. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3344-0_6
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DOI: https://doi.org/10.1007/978-1-4939-3344-0_6
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