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Superantigens pp 67–71Cite as

Lipopolysaccharide-Induced Toxic Shock Syndrome in Rabbits

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1396))

Abstract

Enhancement of susceptibility to lipopolysaccharide (LPS; endotoxin) is a defining characteristic of Staphylococcus aureus superantigens. At the time of this publication, there are 24 identified staphylococcal superantigens (SAgs), some of which have yet to be fully characterized. Testing the capacity of superantigens to potentiate LPS sensitivity is essential to characterize the role of these proteins in disease development. Here we describe how to perform studies of the enhancement of LPS-induced toxic shock syndrome in rabbits. This protocol also provides information on a second important activity of superantigens: the production of fever.

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Author information

Authors and Affiliations

  1. Department of Microbiology, University of Iowa, 51 Newton Road, Bowen Science Building 3-403, Iowa City, IA, 52242, USA

    Christopher S. Stach & Patrick M. Schlievert

Authors
  1. Christopher S. Stach
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  2. Patrick M. Schlievert
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Corresponding author

Correspondence to Patrick M. Schlievert .

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Editors and Affiliations

  1. Science Department, Concordia University - Saint Paul, St. Paul, Minnesota, USA

    Amanda J. Brosnahan

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Stach, C.S., Schlievert, P.M. (2016). Lipopolysaccharide-Induced Toxic Shock Syndrome in Rabbits. In: Brosnahan, A. (eds) Superantigens. Methods in Molecular Biology, vol 1396. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3344-0_5

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  • DOI: https://doi.org/10.1007/978-1-4939-3344-0_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3342-6

  • Online ISBN: 978-1-4939-3344-0

  • eBook Packages: Springer Protocols

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