Abstract
Studies on bacterial enterotoxin-epithelium interactions require model systems capable of mimicking the events occurring at the molecular and cellular levels during intoxication. In this chapter, we describe organ culture as an often neglected alternative to whole-animal experiments or enterocyte-like cell lines. Like cell culture, organ culture is versatile and suitable for studying rapidly occurring events, such as enterotoxin binding and uptake. In addition, it is advantageous in offering an epithelium with more authentic permeability/barrier properties than any cell line, as well as a subepithelial lamina propria, harboring the immune cells of the gut mucosa.
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Acknowledgements
The work was supported by grants from Augustinus Fonden, Aase og Ejnar Danielsens Fond, Brødrene Hartmanns Fond, Fonden til Lægevidenskabens Fremme, and Hørslev Fonden. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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Lorenzen, U.S., Hansen, G.H., Danielsen, E.M. (2016). Organ Culture as a Model System for Studies on Enterotoxin Interactions with the Intestinal Epithelium. In: Brosnahan, A. (eds) Superantigens. Methods in Molecular Biology, vol 1396. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3344-0_14
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DOI: https://doi.org/10.1007/978-1-4939-3344-0_14
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