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From Phosphoproteome to Modeling of Plant Signaling Pathways

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Proteomics in Systems Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1394))

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Abstract

Quantitative proteomic experiments in recent years became almost routine in many aspects of biology. Particularly the quantification of peptides and corresponding phosphorylated counterparts from a single experiment is highly important for understanding of dynamics of signaling pathways. We developed an analytical method to quantify phosphopeptides (pP) in relation to the quantity of the corresponding non-phosphorylated parent peptides (P). We used mixed-mode solid-phase extraction to purify total peptides from tryptic digest and separated them from most of the phosphorous-containing compounds (e.g., phospholipids, nucleotides) which enhances pP enrichment on TiO2 beads. Phosphoproteomic data derived with this designed method allows quantifying pP/P stoichiometry, and qualifying experimental data for mathematical modeling.

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Authors and Affiliations

  1. Plant Systems Biology, Plant Physiology, University of Hohenheim, Fruwirthstrasse 12, 70599, Stuttgart, Germany

    Maksim Zakhartsev, Heidi Pertl-Obermeyer & Waltraud X. Schulze

Authors
  1. Maksim Zakhartsev
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  2. Heidi Pertl-Obermeyer
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  3. Waltraud X. Schulze
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Corresponding author

Correspondence to Maksim Zakhartsev .

Editor information

Editors and Affiliations

  1. University of Regensburg Institute of Functional Genomics, Regensburg, Germany

    Jörg Reinders

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Zakhartsev, M., Pertl-Obermeyer, H., Schulze, W.X. (2016). From Phosphoproteome to Modeling of Plant Signaling Pathways. In: Reinders, J. (eds) Proteomics in Systems Biology. Methods in Molecular Biology, vol 1394. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3341-9_18

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  • DOI: https://doi.org/10.1007/978-1-4939-3341-9_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3339-6

  • Online ISBN: 978-1-4939-3341-9

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