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Chimeric Antigen Receptors for Cancer Immunotherapy

  • Claudia Geldres
  • Barbara Savoldo
  • Gianpietro Dotti
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1393)

Abstract

The adoptive transfer of T lymphocytes expressing chimeric antigen receptors (CARs) has emerged as a promising treatment for various lymphoid and solid malignancies. Patients treated with CAR-T cells have achieved dramatic responses and in some cases, complete tumor eradication. Given that CARs combine the specificity of a monoclonal antibody with the internal signaling domains of T cells, there is flexibility for choice of target antigen and strength of T-cell activation. This targeting mechanism also relinquishes the need for antigen processing and presentation via the major histocompatibility complex (MHC), making CARs a very attractive therapeutic option for the majority of patients. This review describes current methodological strategies used to generate CAR molecules, how to insert these molecules in T lymphocytes and how to evaluate the functionality of these CAR-T cells using various in vitro and in vivo experiments.

Key words

Immunotherapy Chimeric antigen receptor 

Notes

Acknowledgements

This work was supported in part by R01 CA142636 National Institutes of Health-NCI, W81XWH-10-10425 Department of Defence and Technology/Therapeutic Development Award.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Claudia Geldres
    • 1
    • 2
  • Barbara Savoldo
    • 1
    • 3
    • 4
  • Gianpietro Dotti
    • 1
    • 2
    • 4
    • 5
    • 6
    • 7
    • 8
  1. 1.Center for Cell and Gene TherapyBaylor College of MedicineHoustonUSA
  2. 2.Interdepartmental Program in Translational Biology and Molecular MedicineBaylor College of MedicineHoustonUSA
  3. 3.Department of PediatricsBaylor College of MedicineHoustonUSA
  4. 4.Texas Children’s HospitalHoustonUSA
  5. 5.Departments of PathologyBaylor College of MedicineHoustonUSA
  6. 6.Departments of ImmunologyBaylor College of MedicineHoustonUSA
  7. 7.Department of MedicineBaylor College of MedicineHoustonUSA
  8. 8.Houston Methodist HospitalHoustonUSA

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