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Natural Antibodies to Tumor-Associated Antigens

  • Sheila María Álvarez-Fernández
  • Lucia De Monte
  • Massimo Alessio
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1393)

Abstract

Natural autoantibodies raised by humoral immune response to cancer can be exploited to identify potential tumor-associated antigens (TAAs), and might constitute new putative prognostic and/or diagnostic biomarkers. Here we describe how sera from tumor patients can be used to identify TAAs by screening antibody immunoreactivity against the cancer proteome resolved by two-dimensional gel electrophoresis.

Key words

2D-electrophoresis Immunoproteomics Tumor-associated antigens Autoantibodies Western blot 

Notes

Acknowledgments

M.A. is supported by AIRC. Special Program Molecular Clinical Oncology 5 per mille n.9965, and IG-10538.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Sheila María Álvarez-Fernández
    • 1
  • Lucia De Monte
    • 2
  • Massimo Alessio
    • 1
  1. 1.Proteome Biochemistry UnitIRCCS San Raffaele HospitalMilanoItaly
  2. 2.Tumor Immunology UnitIRCCS San Raffaele HospitalMilanoItaly

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