Abstract
RNA interference (RNAi) is a powerful tool for efficient and highly specific gene silencing. Transduction with lentiviral vectors provides stable and long-term gene expression in slowly and non-dividing cells. This chapter describes how to couple these two technologies to efficiently silence specific genes in primary acute myeloid leukemia (AML) cells for subsequent xenotransplantation in immunocompromised mice. This approach could be used for loss-of-function studies aimed at identifying oncogenic targets in AML.
The original version of this chapter was revised. An erratum to this chapter can be found at DOI 10.1007/978-1-4939-3338-9_16
An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-1-4939-3338-9_16
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di Robilant, B.N., Noviello, M. (2016). Efficient RNA Interference of Primary Leukemic Cells for Loss-of-Function Studies in Xenograft Mouse Models. In: Bondanza, A., Casucci, M. (eds) Tumor Immunology. Methods in Molecular Biology, vol 1393. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3338-9_11
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DOI: https://doi.org/10.1007/978-1-4939-3338-9_11
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3336-5
Online ISBN: 978-1-4939-3338-9
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