Abstract
Engagement of Toll-like receptors (TLRs) on dendritic cells (DCs) triggers the expression of a large set of genes involved in DC activation and maturation, which allow them to act efficiently as antigen-presenting cells. Recently, it has become clear that TLR signalling in DCs also results in dramatic metabolic changes that are integral to their changed biology. Here, we describe a detailed protocol on how DC metabolism can be studied after TLR stimulation using the 96-well format Extracellular Flux (XFe96) Analyzer from Seahorse Bioscience, a machine that allows one to simultaneously assess rates of oxidative phosphorylation and glycolysis in real-time, in live cells and in a high-throughput manner.
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Pelgrom, L.R., van der Ham, A.J., Everts, B. (2016). Analysis of TLR-Induced Metabolic Changes in Dendritic Cells Using the Seahorse XFe96 Extracellular Flux Analyzer. In: McCoy, C. (eds) Toll-Like Receptors. Methods in Molecular Biology, vol 1390. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3335-8_17
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DOI: https://doi.org/10.1007/978-1-4939-3335-8_17
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3333-4
Online ISBN: 978-1-4939-3335-8
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