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Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, Second Edition pp 367–385Cite as

Protocols for Improvement of Black Pepper (Piper nigrum L.) Utilizing Biotechnological Tools

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1391))

Abstract

Black pepper, Piper nigrum L., the “King of spices” is the most widely used spice growing in the South-Western region of India. The humid tropical evergreen forest bordering the Malabar Coast (Western Ghats is one of the hot spot areas of plant bio-diversity on earth) is its center of origin and diversity. However, the crop faces constraints like rampant fungal and viral diseases, lack of disease free planting material, hence biotechnological tools can be utilized to address these problems and strides have been made successfully. The standardization of micropropagation, somatic embryogenesis, in vitro conservation, protoplast isolation, and genetic transformation protocols are described here. The protocols could be utilized to achieve similar goals in the related species of Piper too.

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Abbreviations

BA:

Benzyl adenine

IBA :

Indole-3-butyric acid

Kin:

Kinetin

MS:

Murashige and Skoog

NAA :

α-naphthalene acetic acid

SH:

Schenk and Hildebrandt

TDZ :

Thiaduzuron

WPM:

Woody plant medium

References

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Author information

Authors and Affiliations

  1. All India Coordinated Research Project on Spices, Indian Institute of Spices Research, Kozhikode, 673 012, Kerala, India

    K. Nirmal Babu, G. Yamuna & P. N. Ravindran

  2. Providence Women’s College, Kozhikode, 673 009, India

    Minoo Divakaran

  3. World Noni Research Foundation, Chennai, 600 020, India

    K. V. Peter

Authors
  1. K. Nirmal Babu
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  2. Minoo Divakaran
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  3. G. Yamuna
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  4. P. N. Ravindran
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  5. K. V. Peter
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Corresponding author

Correspondence to K. Nirmal Babu .

Editor information

Editors and Affiliations

  1. Applied Biology, Univ of Helsinki, Helsinki, Finland

    S.Mohan Jain

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© 2016 Springer Science+Business Media New York

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Nirmal Babu, K., Divakaran, M., Yamuna, G., Ravindran, P.N., Peter, K.V. (2016). Protocols for Improvement of Black Pepper (Piper nigrum L.) Utilizing Biotechnological Tools. In: Jain, S. (eds) Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, Second Edition. Methods in Molecular Biology, vol 1391. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3332-7_26

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  • DOI: https://doi.org/10.1007/978-1-4939-3332-7_26

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3330-3

  • Online ISBN: 978-1-4939-3332-7

  • eBook Packages: Springer Protocols

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