Abstract
Plants are natural sources of valuable secondary metabolites used as pharmaceuticals, agrochemicals, flavors, fragrances, colors, biopesticides, and food additives. There is an increasing demand to obtain these metabolites through more productive plant tissue applications and cell culture methods due to the importance of secondary metabolites.
Immobilization of plant cells is a method used in plant cell cultures to induce secondary metabolite production. In this method, plant cells are fixed in or on a supporting material or matrix such as agar, agarose, calcium alginate, glass, or polyurethane foam. In the present study, three natural lignocellulosic materials, loofah sponge, and the long fibers of sisal and jute, were used to immobilize suspended R. tinctorum cells.
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References
Brodelius P, Mosbach K (1982) Immobilized plant cells. Appl Biochem Biotechnol 7:31–34
Premjet D, Tachibana S (2004) Production of podophyllotoxin by immobilized cell cultures of Juniperus chinensis. Pak J Biol Sci 7(7):1130–1134
Mavituna F, Park JM (1985) Growth of immobilised plant cells in reticulate polyurethane foam matrices. Biotechnol Lett 7(9):637–640
Soderquist R, Lee MJ (2005) Plant cell immobilisation applications. In: Nedovic V, Willaert R (eds.) Applications of cell immobilisation technology. Hofman M, Anne J (Series eds.). Springer, Dordrecht, Berlin, Heidelberg, New York, pp 469–477
Facchini PJ, DiCosmo F (1991) Secondary metabolite biosynthesis in cultured cells of Catharanthus roseus (L.) G. Don immobilized by adhesion to glass fibres. Appl Microbiol Biotechnol 35:382–392
Facchini PJ, DiCosmo F (1991) Plant cell bioreactor for the production of protoberberine alkaloids from immobilized Thalictrum rugosum cultures. Biotechnol Bioeng 37(5):397–403
Campostrini R, Carturan G, Caniato R, Piovan A, Filippini R, Innocenti G, Cappelletti EM (1996) Immobilization of plant cells in hybrid sol–gel materials. J Sol-Gel Sci Technol 7:87–97
Nartop P, Akay Ş, Gürel A (2013) Immobilization of Rubia tinctorum L. suspension cultures and its effects on alizarin and purpurin accumulation and biomass production. Plant Cell Tiss Org 112(1):123–128
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–497
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Nartop, P. (2016). Immobilization of Rubia tinctorum L. Suspension Cultures and Biomass Production. In: Jain, S. (eds) Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, Second Edition. Methods in Molecular Biology, vol 1391. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3332-7_10
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DOI: https://doi.org/10.1007/978-1-4939-3332-7_10
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