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Principles of Amnis Imaging Flow Cytometry

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Imaging Flow Cytometry

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1389))

Abstract

Over the last decade imaging flow cytometry (IFC) has become an established technique, as evidenced by its use in over 500 peer-reviewed scientific articles. Nevertheless, it is still an emerging technique with an installed base of less than 5 % that of conventional flow cytometers. In parallel with its adoption, the technology has evolved rapidly, increasing in speed, sensitivity, and real-time data analysis capacity by over an order of magnitude since its introduction. This chapter summarizes IFC’s basic principles of operation and describes the current state of the art.

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Author information

Authors and Affiliations

  1. Department of Bioengineering, University of Washington, Seattle, WA, USA

    David A. Basiji

Authors
  1. David A. Basiji
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Correspondence to David A. Basiji .

Editor information

Editors and Affiliations

  1. Cellular and Molecular Medicine Program, Boston Children’s Hospital, Boston, Massachusetts, USA

    Natasha S. Barteneva

  2. A.N. Belozersky Institute of Physico-Che, M.V. Lomonosov Moscow State University, Moscow, Russia

    Ivan A. Vorobjev

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Basiji, D.A. (2016). Principles of Amnis Imaging Flow Cytometry. In: Barteneva, N., Vorobjev, I. (eds) Imaging Flow Cytometry. Methods in Molecular Biology, vol 1389. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3302-0_2

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  • DOI: https://doi.org/10.1007/978-1-4939-3302-0_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3300-6

  • Online ISBN: 978-1-4939-3302-0

  • eBook Packages: Springer Protocols

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