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Imaging Flow Cytometric Analysis of Primary Bone Marrow Megakaryocytes

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Book cover Imaging Flow Cytometry

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1389))

Abstract

In light of the indispensible role of platelets in the maintenance of hemostasis, understanding the biology of platelet production from bone marrow megakaryocytes (MKs) may uncover new therapeutic strategies for thrombocytopenia. While there has been much recent interest in optimizing culture systems to facilitate the study of the morphologically unique MK lineage, these systems lack the intricacy of in vivo megakaryopoiesis. Given the limitations of many common techniques for the in vivo study of MKs, in this chapter we describe a method to quantify and analyze primary murine bone marrow megakaryocytes utilizing imaging flow cytometry.

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Acknowledgements

The authors gratefully acknowledge the technical support of Dr. Tim Bushnell and the Flow Cytometry Core Facility at the University of Rochester Medical Center. The development of this methodology was supported by funding from the National Institute of Digestive and Kidney Diseases (NIDDK) and the National Institute of Allergy and Infectious Diseases (NIAID) of the National Institutes of Health.

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Authors and Affiliations

  1. Department of Pediatrics, Center for Pediatric Biomedical Research, University of Rochester Medical Center, Box 703, 601 Elmwood Avenue, Rochester, NY, 14642, USA

    Lisa M. Niswander, James Palis & Kathleen E. McGrath

  2. Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY, USA

    Lisa M. Niswander & James Palis

Authors
  1. Lisa M. Niswander
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  2. James Palis
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  3. Kathleen E. McGrath
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Corresponding author

Correspondence to Kathleen E. McGrath .

Editor information

Editors and Affiliations

  1. Cellular and Molecular Medicine Program, Boston Children’s Hospital, Boston, Massachusetts, USA

    Natasha S. Barteneva

  2. A.N. Belozersky Institute of Physico-Che, M.V. Lomonosov Moscow State University, Moscow, Russia

    Ivan A. Vorobjev

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Niswander, L.M., Palis, J., McGrath, K.E. (2016). Imaging Flow Cytometric Analysis of Primary Bone Marrow Megakaryocytes. In: Barteneva, N., Vorobjev, I. (eds) Imaging Flow Cytometry. Methods in Molecular Biology, vol 1389. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3302-0_19

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  • DOI: https://doi.org/10.1007/978-1-4939-3302-0_19

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3300-6

  • Online ISBN: 978-1-4939-3302-0

  • eBook Packages: Springer Protocols

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