Abstract
Analysis of granulocyte function can provide important information about the state of the body’s innate immune system. Existing flow cytometry methods that lack image-based analysis capabilities fail to fully evaluate granulocyte function. In the present method, we combine simultaneous detection of phagocytosis and oxidative burst in granulocytes to identify unique subsets of activated granulocytes. This analysis method provides novel information about granulocytes that allows our lab and others to evaluate the effectiveness of nutritional and lifestyle countermeasures, designed to improve immunity.
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Acknowledgements
The present study was funded in part by a Research Initiation Grant (RIG) from the University of North Texas to Dr. McFarlin. The authors did not receive direct funding for the completion of this study and report no conflict of interest.
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McFarlin, B.K., Venable, A.S., Henning, A.L., Williams, R.R., Prado, E.A. (2016). Assessment of Granulocyte Subset Activation: New Information from Image-Based Flow Cytometry. In: Barteneva, N., Vorobjev, I. (eds) Imaging Flow Cytometry. Methods in Molecular Biology, vol 1389. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3302-0_12
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DOI: https://doi.org/10.1007/978-1-4939-3302-0_12
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