Abstract
Joining an established electrophysiology laboratory typically means that the micropipette fabrication process has already been perfected allowing newcomers to be trained without a complete understanding of how the current method was developed. This chapter looks to fill that knowledge gap by discussing available micropipette options and fabrication methods currently used for in vitro electrophysiology (e.g., inside-out, outside-out, whole-cell, cell-attached, and sharp-electrode patches). We also discuss the importance of the micropipette’s shape in relation to the in vitro electrophysiology model used (e.g., tissue slice, cell culture, dissociated neurons). For laboratories setting-up electrophysiology equipment, we have provided information regarding tools needed for pulling micropipettes as well as post-pulling fabrication processes (e.g., fire polishing). Finally, we discuss micropipette drift, which can occur during electrophysiological recordings accompanied by a micropipette-drift troubleshooting guide. Surprisingly, the process of micropipette fabrication can play a significant role in data collection. With this in mind, we anticipate by the end of this chapter that the reader will have the information necessary to fabricate the appropriate micropipette for their desired electrophysiology preparation allowing for successful data collection.
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References
Levis R, Rae J (2002) Technology of patch-clamp electrodes. In: Walz W, Boulton A, Baker G (eds) Patch-clamp analysis, 35. Humana, Totowa, pp 1–34
Rae JL, Levis RA (2004) Fabrication of patch pipets. Curr Protoc Neurosci. Chapter 6:Unit 6.3
Copello J, Simon B, Segal Y, Wehner F, Ramanujam VM, Alcock N, Reuss L (1991) Ba2+ release from soda glass modifies single maxi K+ channel activity in patch clamp experiments. Biophys J 60(4):931–941
Furman RE, Tanaka JC (1988) Patch electrode glass composition affects ion channel currents. Biophys J 53(2):287–292
Oesterle A (2008) P-97 pipette cookbook. Sutter Instruments, Novato, CA
Brown AL, Johnson BE, Goodman MB (2008) Making patch-pipettes and sharp electrodes with a programmable puller. J Vis Exp(20)
Goodman MB, Lockery SR (2000) Pressure polishing: a method for re-shaping patch pipettes during fire polishing. J Neurosci Methods 100(1–2):13–15
Johnson BE, Brown AL, Goodman MB (2008) Pressure-polishing pipettes for improved patch-clamp recording. J Vis Exp(20)
Ogden D, Stanfield P (1994) Patch clamp techniques for single channel and whole-cell recording Microelectrode techniques: the Plymouth workshop handbook. Company of Biologists, Cambridge, UK
Sachs F (1995) A low drift micropipette holder. Pflugers Arch 429(3):434–435
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Graziane, N., Dong, Y. (2016). Patch Pipettes (Micropipettes). In: Electrophysiological Analysis of Synaptic Transmission. Neuromethods, vol 112. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3274-0_5
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DOI: https://doi.org/10.1007/978-1-4939-3274-0_5
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3273-3
Online ISBN: 978-1-4939-3274-0
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