Abstract
In order to develop physiologically relevant experiments that test electrical activity in and between neurons, it is necessary to closely model the physiological milieu. This chapter discusses external and internal solution components for in vitro brain slice electrophysiology. These recipes have been developed throughout the years to model the physiological environment and to help produce viable, healthy neurons.
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Polderman KH, van de Kraats G, Dixon JM, Vandertop WP, Girbes AR (2003) Increases in spinal fluid osmolarity induced by mannitol. Crit Care Med 31(2):584–590
Sharp PE, Regina MCL (1998) The laboratory rat. Taylor & Francis, Boston, MA
Irani DN (2009) Cerebrospinal fluid in clinical practice. Saunders/Elsevier, Philadelphia, PA
Reed D, Withrow CD, Woodbury D (1967) Electrolyte and acid-base parameters of rat cerebrospinal fluid. Exp Brain Res 3(3):212–219
Jeong SM, Hahm KD, Shin JW, Leem JG, Lee C, Han SM (2006) Changes in magnesium concentration in the serum and cerebrospinal fluid of neuropathic rats. Acta Anaesthesiol Scand 50(2):211–216
LeVine SM, Wulser MJ, Lynch SG (1998) Iron quantification in cerebrospinal fluid. Anal Biochem 265(1):74–78
Espino A, Ambrosio S, Bartrons R, Bendahan G, Calopa M (1994) Cerebrospinal monoamine metabolites and amino acid content in patients with parkinsonian syndrome and rats lesioned with MPP+. J Neural Transm Park Dis Dement Sect 7(3):167–176
Ganrot K, Laurell C-B (1974) Measurement of IgG and albumin content of cerebrospinal fluid, and its interpretation. Clin Chem 20(5):571–573
Habgood MD, Sedgwick JE, Dziegielewska KM, Saunders NR (1992) A developmentally regulated blood-cerebrospinal fluid transfer mechanism for albumin in immature rats. J Physiol 456:181–192
Hutchesson A, Preece MA, Gray G, Green A (1997) Measurement of lactate in cerebrospinal fluid in investigation of inherited metabolic disease. Clin Chem 43(1):158–161
Swahn CG, Sedvall G (1988) CSF creatinine in schizophrenia. Biol Psychiatry 23(6):586–594
Martina M, Taverna S (2014) Patch-clamp methods and protocols. Springer, New York
Huang S, Uusisaari MY (2013) Physiological temperature during brain slicing enhances the quality of acute slice preparations. Front Cell Neurosci 7:48
Lipton P, Aitken PG, Dudek FE, Eskessen K, Espanol MT, Ferchmin PA, Kelly JB, Kreisman NR, Landfield PW, Larkman PM et al (1995) Making the best of brain slices: comparing preparative methods. J Neurosci Methods 59(1):151–156
Lee BR, Ma YY, Huang YH, Wang X, Otaka M, Ishikawa M, Neumann PA, Graziane NM, Brown TE, Suska A, Guo C, Lobo MK, Sesack SR, Wolf ME, Nestler EJ, Shaham Y, Schluter OM, Dong Y (2013) Maturation of silent synapses in amygdala-accumbens projection contributes to incubation of cocaine craving. Nat Neurosci 16(11):1644–1651
Alvarez-Leefmans F (1990) Intracellular Cl− regulation and synaptic inhibition in vertebrate and invertebrate neurons. In: Alvarez-Leefmans F, Russell J (eds) Chloride channels and carriers in nerve, muscle, and glial cells. Springer, USA, pp 109–158
Chesler M (1990) The regulation and modulation of pH in the nervous system. Prog Neurobiol 34(5):401–427
Erecinska M, Silver IA (1989) ATP and brain function. J Cereb Blood Flow Metab 9(1):2–19
Taylor JS, Vigneron DB, Murphy-Boesch J, Nelson SJ, Kessler HB, Coia L, Curran W, Brown TR (1991) Free magnesium levels in normal human brain and brain tumors: 31P chemical-shift imaging measurements at 1.5 T. Proc Natl Acad Sci U S A 88(15):6810–6814
Higashijima T, Ferguson KM, Sternweis PC (1987) Regulation of hormone-sensitive GTP-dependent regulatory proteins by chloride. J Biol Chem 262(8):3597–3602
Sarantopoulos C (2007) Perforated patch-clamp techniques. Neuromethods 38:253–293
Kay AR (1992) An intracellular medium formulary. J Neurosci Methods 44(2–3):91–100
Liao D, Hessler NA, Malinow R (1995) Activation of postsynaptically silent synapses during pairing-induced LTP in CA1 region of hippocampal slice. Nature 375(6530):400–404
Kettenmann H, Grantyn R (1992) Practical electrophysiological methods: a guide for in vitro studies in vertebrate neurobiology. Wiley, Chichester
Belles B, Malécot CO, Hescheler J, Trautwein W (1988) “Run-down” of the Ca current during long whole-cell recordings in guinea pig heart cells: role of phosphorylation and intracellular calcium. Pflugers Arch 411(4):353–360
Horn R, Korn SJ (1992) Prevention of rundown in electrophysiological recording. Methods Enzymol 207:149–155
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Graziane, N., Dong, Y. (2016). Salt Environment. In: Electrophysiological Analysis of Synaptic Transmission. Neuromethods, vol 112. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3274-0_4
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DOI: https://doi.org/10.1007/978-1-4939-3274-0_4
Publisher Name: Humana Press, New York, NY
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