Salt Environment

  • Nicholas GrazianeEmail author
  • Yan Dong
Part of the Neuromethods book series (NM, volume 112)


In order to develop physiologically relevant experiments that test electrical activity in and between neurons, it is necessary to closely model the physiological milieu. This chapter discusses external and internal solution components for in vitro brain slice electrophysiology. These recipes have been developed throughout the years to model the physiological environment and to help produce viable, healthy neurons.

Key words

Ionic concentrations Brain slice preparation Osmolality Osmolarity 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Neuroscience DepartmentUniversity of PittsburghPittsburghUSA

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