Abstract
So far we have discussed patch clamp procedures that target the neuronal soma. However, electrical properties of neurons are critically influenced by dendrites, which not only make up a large portion of neuron’s surface area and but also receive a large proportion of synaptic inputs. The electrical properties of dendritic membrane and somatic membrane can be vey different. Therefore, recording electrical properties directly from dendrites is desired in some experimental designs. Luckily, skilled electrophysiologists have optimized electrophysiological protocols that allow neuronal dendritic recording. Such recordings have provided important information regarding voltage-gated ion channel distribution and function [1–5], differences between somatic and dendritic membrane channel properties [6, 7], and synaptic integration of backpropagating action potentials combined with synaptic potentials [8–10]. The purpose of this chapter is to provide the beginning electrophysiologist with the necessary information required to maximize their success rate for dendritic recordings. While reading this chapter, we also would like to refer the reader to a superb detailed approach to dendritic patching documented by [11].
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Bekkers JM (2000) Distribution and activation of voltage-gated potassium channels in cell-attached and outside-out patches from large layer 5 cortical pyramidal neurons of the rat. J Physiol 525(Pt 3):611–620
Hoffman DA, Magee JC, Colbert CM, Johnston D (1997) K+ channel regulation of signal propagation in dendrites of hippocampal pyramidal neurons. Nature 387(6636):869–875
Korngreen A, Sakmann B (2000) Voltage-gated K+ channels in layer 5 neocortical pyramidal neurones from young rats: subtypes and gradients. J Physiol 525(Pt 3):621–639
Magee JC (1998) Dendritic hyperpolarization-activated currents modify the integrative properties of hippocampal CA1 pyramidal neurons. J Neurosci 18(19):7613–7624
Stuart G, Hausser M (1994) Initiation and spread of sodium action potentials in cerebellar Purkinje cells. Neuron 13(3):703–712
Magee JC, Cook EP (2000) Somatic EPSP amplitude is independent of synapse location in hippocampal pyramidal neurons. Nat Neurosci 3(9):895–903
Williams SR, Stuart GJ (2002) Dependence of EPSP efficacy on synapse location in neocortical pyramidal neurons. Science 295(5561):1907–1910
Magee JC, Johnston D (1997) A synaptically controlled, associative signal for Hebbian plasticity in hippocampal neurons. Science 275(5297):209–213
Stuart GJ, Hausser M (2001) Dendritic coincidence detection of EPSPs and action potentials. Nat Neurosci 4(1):63–71
Watanabe S, Hoffman DA, Migliore M, Johnston D (2002) Dendritic K+ channels contribute to spike-timing dependent long-term potentiation in hippocampal pyramidal neurons. Proc Natl Acad Sci U S A 99(12):8366–8371
Davie JT, Kole MH, Letzkus JJ, Rancz EA, Spruston N, Stuart GJ, Hausser M (2006) Dendritic patch-clamp recording. Nat Protoc 1(3):1235–1247
Larkum ME, Senn W, Luscher HR (2004) Top-down dendritic input increases the gain of layer 5 pyramidal neurons. Cereb Cortex 14(10):1059–1070
Nevian T, Larkum ME, Polsky A, Schiller J (2007) Properties of basal dendrites of layer 5 pyramidal neurons: a direct patch-clamp recording study. Nat Neurosci 10(2):206–214
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
Copyright information
© 2016 Springer Science+Business Media New York
About this protocol
Cite this protocol
Graziane, N., Dong, Y. (2016). Dendritic Patch. In: Electrophysiological Analysis of Synaptic Transmission. Neuromethods, vol 112. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3274-0_20
Download citation
DOI: https://doi.org/10.1007/978-1-4939-3274-0_20
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3273-3
Online ISBN: 978-1-4939-3274-0
eBook Packages: Springer Protocols