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Expression of Multiple Functional RNAs or Proteins from One Viral Vector

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Gene Therapy for Neurological Disorders

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1382))

Abstract

In this chapter, we will cover the available design choices for enabling expression of two functional protein or RNA sequences from a single viral vector. Such vectors are very useful in the neuroscience-related field of neuronal control and modulation, e.g., using optogenetics or DREADDs, but are also desirable in applications of CRISPR/Cas9 in situ genome editing and more refined therapeutic approaches. Each approach to achieving this combined expression has its own strengths and limitations, which makes them more or less suitable for different applications. In this chapter, we describe the available alternatives and provide tips on how they can be implemented.

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Authors and Affiliations

  1. Molecular Neuromodulation, Wallenberg Neuroscience Center, Lund University, 117, 221 00, Lund, Sweden

    Tomas Björklund

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  1. Tomas Björklund
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Correspondence to Tomas Björklund .

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Editors and Affiliations

  1. Translational Science and Molecular Medicine, Michigan State University, College of Human Science, Grand Rapids, Michigan, USA

    Fredric P. Manfredsson

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Björklund, T. (2016). Expression of Multiple Functional RNAs or Proteins from One Viral Vector. In: Manfredsson, F. (eds) Gene Therapy for Neurological Disorders. Methods in Molecular Biology, vol 1382. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3271-9_3

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  • DOI: https://doi.org/10.1007/978-1-4939-3271-9_3

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3270-2

  • Online ISBN: 978-1-4939-3271-9

  • eBook Packages: Springer Protocols

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