Abstract
Stereotaxic surgery is an invaluable tool to deliver a variety of gene therapy constructs to the nonhuman primate caudate and putamen in preclinical studies for the genetic, neurodegenerative disorder, Huntington’s disease (HD). Here we describe in detail how to perform this technique beginning with a pre-surgical magnetic resonance imaging scan to determine surgical coordinates followed by the stereotaxic surgical injection technique. In addition, we include methodology of a full necropsy including brain and peripheral tissue removal and a standard immunohistochemical technique to visualize the injected gene therapy agent.
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Acknowledgements
This work was supported by NIH Grant NS069798 (JLM), and Oregon National Primate Research Center (ONPRC) Core Grants RR000163 and RR000163.
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McBride, J.L., Clark, R.L. (2016). Stereotaxic Surgical Targeting of the Nonhuman Primate Caudate and Putamen: Gene Therapy for Huntington’s Disease. In: Manfredsson, F. (eds) Gene Therapy for Neurological Disorders. Methods in Molecular Biology, vol 1382. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3271-9_29
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DOI: https://doi.org/10.1007/978-1-4939-3271-9_29
Publisher Name: Humana Press, New York, NY
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