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Gene Therapy for Neurological Disorders pp 251–261Cite as

AAV-Mediated Gene Transfer to Dorsal Root Ganglion

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1382))

Abstract

Transferring genetic molecules into the peripheral sensory nervous system to manipulate nociceptive pathophysiology is a powerful approach for experimental modulation of sensory signaling and potentially for translation into therapy for chronic pain. This can be efficiently achieved by the use of recombinant adeno-associated virus (rAAV) in conjunction with nociceptor-specific regulatory transgene cassettes. Among different routes of delivery, direct injection into the dorsal root ganglia (DRGs) offers the most efficient AAV-mediated gene transfer selectively into the peripheral sensory nervous system. Here, we briefly discuss the advantages and applications of intraganglionic microinjection, and then provide a detailed approach for DRG injection, including a list of the necessary materials and description of a method for performing DRG microinjection experiments. We also discuss our experience with several adeno-associated virus (AAV) options for in vivo transgene expression in DRG neurons.

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Acknowledgement

This work was supported by grants from the Department of Veterans Affairs Rehabilitation Research and Development (3690-03), the Advancing a Healthier Wisconsin (FP00005706), and the National Institute of Neurological Disorders and Stroke (R01NS079626-01), to Q.H.H.

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Authors and Affiliations

  1. Department of Anesthesiology, Medical College of Wisconsin, Watertown Plank Road, Milwaukee, WI, 53226, USA

    Hongwei Yu M.D., Gregory Fischer & Quinn H. Hogan

Authors
  1. Hongwei Yu M.D.
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  2. Gregory Fischer
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  3. Quinn H. Hogan
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Corresponding author

Correspondence to Hongwei Yu M.D. .

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Editors and Affiliations

  1. Translational Science and Molecular Medicine, Michigan State University, College of Human Science, Grand Rapids, Michigan, USA

    Fredric P. Manfredsson

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Yu, H., Fischer, G., Hogan, Q.H. (2016). AAV-Mediated Gene Transfer to Dorsal Root Ganglion. In: Manfredsson, F. (eds) Gene Therapy for Neurological Disorders. Methods in Molecular Biology, vol 1382. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3271-9_18

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  • DOI: https://doi.org/10.1007/978-1-4939-3271-9_18

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3270-2

  • Online ISBN: 978-1-4939-3271-9

  • eBook Packages: Springer Protocols

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