Spot Matching of 2-DE Images Using Distance, Intensity, and Pattern Information

  • Hua-Mei Xin
  • Yuemin ZhuEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1384)


The analysis of a large number of two-dimensional gel electrophoresis (2-DE) images requires developing automatic methods. In such analyses, spot matching plays a fundamental role, in particular for the identification of proteins. We describe a simple and accurate method which allows to automatically and accurately match spots in 2-DE images. The method consists of simultaneously exploiting the distance between the spots, their intensity, and the pattern formed by their spatial configuration.

Key words

Spot matching Spot registration Two-dimensional gel electrophoresis 2-DE images Image registration Proteomics 



The authors thank the team of Dr. Long-Fei WU, Laboratory of Bacterial Chemistry, CNRS URP9034, Marseille, France, for providing the 2-DE images and the ground truth. Yuemin ZHU was partly supported by the French ANR under ANR-13-MONU-0009-01. Hua-Mei Xin was partly supported by the Scientific Research Foundation for the Returned Overseas Chinese Scholars (2009- 36).


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.College of Physics & ElectronicsShandong Normal UniversityShandongChina
  2. 2.CREATIS, CNRS UMR 5220, Inserm U1044, INSA LyonUniversity of LyonVilleurbanne cedexFrance

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