Two-Dimensional Gel Electrophoresis Image Analysis via Dedicated Software Packages
Analyzing two-dimensional gel electrophoretic images is supported by a number of freely and commercially available software. Although the respective program is highly specific, all the programs follow certain standardized algorithms. General steps are: (1) detecting and separating individual spots, (2) subtracting background, (3) creating a reference gel and (4) matching the spots to the reference gel, (5) modifying the reference gel, (6) normalizing the gel measurements for comparison, (7) calibrating for isoelectric point and molecular weight markers, and moreover, (8) constructing a database containing the measurement results and (9) comparing data by statistical and bioinformatic methods.
Key wordsProteomics Two-dimensional gel electrophoresis 2-D GE Bioinformatics Image analysis 2-D software
- 2-D GE
Two-dimensional gel electrophoresis
Analysis of variance
Tagged image file format
The author has been supported by grants of the European Union within the Framework Programme 7, the German Ministry of Research and Education (BMBF) within the National Genome Research Network (NGFN-2), the German Research Foundation DFG, the intramural program of the Medical Faculty of the University of Heidelberg, the Steuben-Schurz Society, and the Estate of Friedrich Fischer. The author thanks Professor em. Dr. Wolfgang Kuschinsky, Heidelberg, for his sustained support and dedicates this work to his 70th birthday.
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