A Simple Liquid Chromatography Tandem Mass Spectrometry Method for Quantitation of Plasma Busulfan

  • Shuang Deng
  • Michael Kiscoan
  • Clint Frazee
  • Susan Abdel-Rahman
  • Jignesh Dalal
  • Uttam GargEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1383)


Busulfan is an alkylating agent widely used in the ablation of bone marrow cells before hematopoietic stem cell transplant. Due to large intraindividual and interindividual variations, and narrow therapeutic window, therapeutic drug monitoring of busulfan is warranted. A quick and reliable HPLC-MS/MS method was developed for the assay of plasma busulfan. HPLC involved C18 column, and MS/MS was used in electrospray ionization (ESI) positive mode. Quantitation and identification of busulfan was made using various multiple reactions monitoring (MRMs). Isotopic labeled busulfan-d8 was used as the internal standard. The method is linear from 50 to 2500 ng/mL and has with-in run and between-run imprecision of <10 %.

Key words

Busulfan Mass spectrometry Liquid chromatography Bone marrow transplant Leukemia 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Shuang Deng
    • 1
    • 2
  • Michael Kiscoan
    • 1
  • Clint Frazee
    • 1
  • Susan Abdel-Rahman
    • 2
  • Jignesh Dalal
    • 2
  • Uttam Garg
    • 1
    Email author
  1. 1.Department of Pathology and Laboratory MedicineChildren’s Mercy Hospitals and ClinicsKansas CityUSA
  2. 2.Department of PediatricsChildren’s Mercy Hospitals and ClinicsKansas CityUSA

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