Simultaneous Quantitation of Lamotrigine, Levetiracetam, 10-Hydroxycarbazepine, Topiramate, and Zonisamide in Serum Using HPLC-MS/MS

  • Dean C. CarlowEmail author
  • Heng Shi
  • Ryan C. Schofield
Part of the Methods in Molecular Biology book series (MIMB, volume 1383)


Antiepileptic drugs (AEDs) are a diverse group of pharmacological agents used in the treatment of epileptic seizures. Over the past several decades some new AEDs, including lamotrigine (LTG), levetiracetam (LVA), oxcarbazepine (OXC), topiramate (TOP), and zonisamide (ZNS), have become widely used. This chapter describes a very simple and rapid liquid chromatography-tandem mass spectrometry method for simultaneous quantitation of LVA, ZNS, LTG, TOP, and MHD in human serum. The method requires a very small amount of serum (50 μL) for multiple drug measurements and has a total analysis time of 4 min that makes it well suited for routine clinical analysis of several drugs simultaneously. The imprecision (CVs) measured at various concentrations across the analytical measurement range (AMR) are less than 7 % for all analytes. The AMR for each analyte is as follows: LVA (1–100 μg/mL), ZNS (0.8–80 μg/mL), TOP (0.5–50 μg/mL), and 0.6–60 μg/mL for LTG and MHD.

Key words

Lamotrigine Levetiracetam 10-Hydroxycarbazepine Topiramate Zonisamide Mass spectrometry Therapeutic drug monitoring Antiepileptic drugs 


  1. 1.
    Schachter SC (1999) Antiepileptic drug therapy: general treatment principles and application for special patient populations. Epilepsia 40(s9):S20–S25CrossRefPubMedGoogle Scholar
  2. 2.
    Neels HM et al (2004) Therapeutic drug monitoring of old and newer anti-epileptic drugs. Clin Chem Lab Med 42(11):1228–1255CrossRefPubMedGoogle Scholar
  3. 3.
    Patsalos PN et al (2008) Antiepileptic drugs—best practice guidelines for therapeutic drug monitoring: a position paper by the subcommission on therapeutic drug monitoring, ILAE Commission on Therapeutic Strategies. Epilepsia 49(7):1239–1276CrossRefPubMedGoogle Scholar
  4. 4.
    Johannessen SI et al (2003) Therapeutic drug monitoring of the newer antiepileptic drugs. Ther Drug Monit 25(3):347–363CrossRefPubMedGoogle Scholar
  5. 5.
    Johannessen SI, Tomson T (2006) Pharmacokinetic variability of newer antiepileptic drugs. Clin Pharmacokinet 45(11):1061–1075CrossRefPubMedGoogle Scholar
  6. 6.
    Perucca E (2000) Is there a role for therapeutic drug monitoring of new anticonvulsants. Clin Pharmacokinet 38(3):191–204CrossRefPubMedGoogle Scholar
  7. 7.
    Krasowski MD (2010) Therapeutic drug monitoring of the newer anti-epilepsy medications. Pharmaceuticals 3(6):1909–1935PubMedCentralCrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of Laboratory MedicineMemorial Sloan Kettering Cancer CenterNew YorkUSA
  2. 2.The Department of Pathology and Laboratory MedicineChildren’sHospital of PhiladelphiaPhiladelphiaUSA

Personalised recommendations