Advertisement

Quantitation of the Oral Anticoagulants Dabigatran, Rivaroxaban, Apixaban, and Warfarin in Plasma Using Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry (UPLC-MS/MS)

  • Jaime H. NoguezEmail author
  • James C. Ritchie
Part of the Methods in Molecular Biology book series (MIMB, volume 1383)

Abstract

This chapter describes a method to measure the oral anticoagulants dabigatran, rivaroxaban, apixaban, and warfarin in plasma samples using ultra-performance liquid chromatography combined with tandem mass spectrometry (UPLC-MS/MS). The instrument is operated in multiple reaction monitoring (MRM) mode with an electrospray ionization (ESI) source in positive ionization mode. Samples are extracted with a 90:10 methanol/0.1 N hydrochloric acid solution containing stable isotope-labeled internal standards for each analyte. After centrifugation the supernatant is transferred to a mass spectrometry vial, injected onto the UPLC-ESI-MS/MS, and quantified using an eight-point calibration curve.

Key words

Anticoagulant Dabigatran Rivaroxaban Apixaban Warfarin Mass spectrometry 

References

  1. 1.
    Gonsalves WI, Pruthi RK, Patnaik MM (2013) The new oral anticoagulants in clinical practice. Mayo Clin Proc 88:495–511CrossRefPubMedGoogle Scholar
  2. 2.
    Stangier J (2008) Clinical pharmacokinetics and pharmacodynamics of the oral direct thrombin inhibitor dabigatran etexilate. Clin Pharmacokinet 47:285–295CrossRefPubMedGoogle Scholar
  3. 3.
    Perzborn E, Roehrig S, Straub A et al (2010) Rivaroxaban: a new oral factor Xa inhibitor. Arterioscler Thromb Vasc Biol 30:376–381CrossRefPubMedGoogle Scholar
  4. 4.
    Raghavan N, Frost CE, Yu Z et al (2009) Apixaban metabolism and pharmacokinetics after oral administration to humans. Drug Metab Dispos 37:74–81CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of PathologyUniversity Hospitals Case Medical CenterClevelandUSA
  2. 2.Department of Pathology and Laboratory MedicineEmory University HospitalAtlantaUSA

Personalised recommendations