Quantitation of the Oral Anticoagulants Dabigatran, Rivaroxaban, Apixaban, and Warfarin in Plasma Using Ultra-Performance Liquid Chromatography with Tandem Mass Spectrometry (UPLC-MS/MS)

  • Jaime H. NoguezEmail author
  • James C. Ritchie
Part of the Methods in Molecular Biology book series (MIMB, volume 1383)


This chapter describes a method to measure the oral anticoagulants dabigatran, rivaroxaban, apixaban, and warfarin in plasma samples using ultra-performance liquid chromatography combined with tandem mass spectrometry (UPLC-MS/MS). The instrument is operated in multiple reaction monitoring (MRM) mode with an electrospray ionization (ESI) source in positive ionization mode. Samples are extracted with a 90:10 methanol/0.1 N hydrochloric acid solution containing stable isotope-labeled internal standards for each analyte. After centrifugation the supernatant is transferred to a mass spectrometry vial, injected onto the UPLC-ESI-MS/MS, and quantified using an eight-point calibration curve.

Key words

Anticoagulant Dabigatran Rivaroxaban Apixaban Warfarin Mass spectrometry 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of PathologyUniversity Hospitals Case Medical CenterClevelandUSA
  2. 2.Department of Pathology and Laboratory MedicineEmory University HospitalAtlantaUSA

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