Determination of Menthol in Plasma and Urine by Gas Chromatography/Mass Spectrometry (GC/MS)
Menthol, a monoterpene, is a principal component of peppermint oil and is used extensively in consumer products as a flavoring aid. It is also commonly used medicinally as a topical skin coolant; to treat inflammation of the mucous membranes, digestive problems, and irritable bowel syndrome (IBS); and in preventing spasms during endoscopy and for its spasmolytic effect on the smooth muscle of the gastrointestinal tract. Menthol has a half life of 3–6 h and is rapidly metabolized to menthol glucuronide which is detectable in urine and serum following menthol use. We describe a method for the determination of total menthol in human plasma and urine using liquid/liquid extraction, gas chromatography/mass spectrometry (GC/MS) in selected ion monitoring mode and menthol-d4 as the internal standard. Controls are prepared with menthol glucuronide and all samples undergo enzymatic hydrolysis for the quantification of total menthol. The method has a linear range of 5–1000 ng/mL, and coefficient of variation <10 %.
Key wordsMenthol GCMS Peppermint oil Irritable bowel syndrome
- 12.Pauwels J, D’Autry W, Van den Bossche L, Dewever C, Forier M, Vandenwaeyenberg S, Wolfs K, Hoogmartens J, Van Schepdael A, Adams E (2012) Optimization and validation of liquid chromatography and headspace-gas chromatography based methods for the quantitative determination of capsaicinoids, salicylic acid, glycol monosalicylate, methyl salicylate, ethyl salicylate, camphor and l-menthol in a topical formulation. J Pharm Biomed Anal 60:51–58CrossRefPubMedGoogle Scholar
- 13.Schulz K, Bertau M, Schlenz K, Malt S, Dressler J, Lachenmeier DW (2009) Headspace solid-phase microextraction-gas chromatography-mass spectrometry determination of the characteristic flavourings menthone, isomenthone, neomenthol and menthol in serum samples with and without enzymatic cleavage to validate post-offence alcohol drinking claims. Anal Chim Acta 646:128–140CrossRefPubMedGoogle Scholar