Abstract
Menthol, a monoterpene, is a principal component of peppermint oil and is used extensively in consumer products as a flavoring aid. It is also commonly used medicinally as a topical skin coolant; to treat inflammation of the mucous membranes, digestive problems, and irritable bowel syndrome (IBS); and in preventing spasms during endoscopy and for its spasmolytic effect on the smooth muscle of the gastrointestinal tract. Menthol has a half life of 3–6 h and is rapidly metabolized to menthol glucuronide which is detectable in urine and serum following menthol use. We describe a method for the determination of total menthol in human plasma and urine using liquid/liquid extraction, gas chromatography/mass spectrometry (GC/MS) in selected ion monitoring mode and menthol-d4 as the internal standard. Controls are prepared with menthol glucuronide and all samples undergo enzymatic hydrolysis for the quantification of total menthol. The method has a linear range of 5–1000 ng/mL, and coefficient of variation <10 %.
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References
Liu B, Fan L, Balakrishna S, Sui A, Morris JB, Jordt SE (2013) TRPM8 is the principal mediator of menthol-induced analgesia of acute and inflammatory pain. Pain 154:2169–2177
Farco JA, Grundmann O (2013) Menthol—pharmacology of an important naturally medicinal “cool”. Mini Rev Med Chem 13:124–131
Kamatou GP, Vermaak I, Viljoen AM, Lawrence BM (2013) Menthol: a simple monoterpene with remarkable biological properties. Phytochemistry 96:15–25
Pittler MH, Ernst E (1998) Peppermint oil for irritable bowel syndrome: a critical review and metaanalysis. Am J Gastroenterol 93:1131–1135
Ford AC, Talley NJ, Spiegel BM, Foxx-Orenstein AE, Schiller L, Quigley EM, Moayyedi P (2008) Effect of fibre, antispasmodics, and peppermint oil in the treatment of irritable bowel syndrome: systematic review and meta-analysis. BMJ 337:a2313
Rozza AL, Hiruma-Lima CA, Takahira RK, Padovani CR, Pellizzon CH (2013) Effect of menthol in experimentally induced ulcers: pathways of gastroprotection. Chem Biol Interact 206:272–278
Gelal A, Jacob P 3rd, Yu L, Benowitz NL (1999) Disposition kinetics and effects of menthol. Clin Pharmacol Ther 66:128–135
Mascher H, Kikuta C, Schiel H (2001) Pharmacokinetics of menthol and carvone after administration of an enteric coated formulation containing peppermint oil and caraway oil. Arzneimittelforschung 51:465–469
Hamasaki K, Kato K, Watanabe T, Yoshimura Y, Nakazawa H, Yamamoto A, Matsunaga A (1998) Determination of l-menthol in pharmaceutical products by high performance liquid chromatography with polarized photometric detection. J Pharm Biomed Anal 16:1275–1280
Kaffenberger RM, Doyle MJ (1990) Determination of menthol and menthol glucuronide in human urine by gas chromatography using an enzyme-sensitive internal standard and flame ionization detection. J Chromatogr 527:59–66
Krzek J, Czekaj JS, Rzeszutko W (2003) Validation of a method for simultaneous determination of menthol and methyl salicylate in pharmaceuticals by capillary gas chromatography with cool on-column injection. Acta Pol Pharm 60:343–349
Pauwels J, D’Autry W, Van den Bossche L, Dewever C, Forier M, Vandenwaeyenberg S, Wolfs K, Hoogmartens J, Van Schepdael A, Adams E (2012) Optimization and validation of liquid chromatography and headspace-gas chromatography based methods for the quantitative determination of capsaicinoids, salicylic acid, glycol monosalicylate, methyl salicylate, ethyl salicylate, camphor and l-menthol in a topical formulation. J Pharm Biomed Anal 60:51–58
Schulz K, Bertau M, Schlenz K, Malt S, Dressler J, Lachenmeier DW (2009) Headspace solid-phase microextraction-gas chromatography-mass spectrometry determination of the characteristic flavourings menthone, isomenthone, neomenthol and menthol in serum samples with and without enzymatic cleavage to validate post-offence alcohol drinking claims. Anal Chim Acta 646:128–140
Spichiger M, Muhlbauer RC, Brenneisen R (2004) Determination of menthol in plasma and urine of rats and humans by headspace solid phase microextraction and gas chromatography—mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 799:111–117
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Peat, J., Frazee, C., Kearns, G., Garg, U. (2016). Determination of Menthol in Plasma and Urine by Gas Chromatography/Mass Spectrometry (GC/MS). In: Garg, U. (eds) Clinical Applications of Mass Spectrometry in Drug Analysis. Methods in Molecular Biology, vol 1383. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3252-8_22
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DOI: https://doi.org/10.1007/978-1-4939-3252-8_22
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3251-1
Online ISBN: 978-1-4939-3252-8
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