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Quantitation of Flecainide, Mexiletine, Propafenone, and Amiodarone in Serum or Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

  • Matthew H. SlawsonEmail author
  • Kamisha L. Johnson-Davis
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1383)

Abstract

Flecainide, mexiletine, propafenone, and amiodarone are antiarrhythmic drugs that are used primarily in the treatment of cardiac arrhythmias. The monitoring of the use of these drugs has applications in therapeutic drug monitoring and overdose situations. LC-MS/MS is used to analyze plasma/serum extracts with loxapine as the internal standard to ensure accurate quantitation and control for any potential matrix effects. Positive ion electrospray is used to introduce the analytes into the mass spectrometer. Selected reaction monitoring of two product ions for each analyte allows for the calculation of ion ratios which ensures correct identification of each analyte, while a matrix matched calibration curve is used for quantitation.

Key words

Flecainide Mexiletine Propafenone Amiodarone Plasma Serum UPLC Mass spectrometry 

References

  1. 1.
    Goodman LS, Brunton LL, Chabner B, Knollmann BC (2011) Goodman & Gilman’s pharmacological basis of therapeutics, 12th edn. McGraw-Hill, New YorkGoogle Scholar
  2. 2.
    Baselt RC (2011) Disposition of toxic drugs and chemicals in man, 9th edn. Biomedical Publications, Seal Beach, CAGoogle Scholar
  3. 3.
    Hofmann U, Pecia M, Heinkele G, Dilger K, Kroemer HK, Eichelbaum M (2000) Determination of propafenone and its phase I and phase II metabolites in plasma and urine by high-performance liquid chromatography-electrospray ionization mass spectrometry. J Chromatogr B Biomed Sci Appl 748(1):113–123CrossRefPubMedGoogle Scholar
  4. 4.
    Yan H, Xiang P, Bo J, Shen M (2007) Determination of mexiletine in human blood by liquid chromatography-tandem mass spectrometry. Fa yi xue za zhi 23(6):441–443PubMedGoogle Scholar
  5. 5.
    Kollroser M, Schober C (2002) Determination of amiodarone and desethylamiodarone in human plasma by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry with an ion trap detector. J Chromatogr B Analyt Technol Biomed Life Sci 766(2):219–226CrossRefPubMedGoogle Scholar
  6. 6.
    Mano Y, Asakawa Y, Kita K, Ishii T, Hotta K, Kusano K (2015) Validation of an ultra-performance liquid chromatography-tandem mass spectrometry method for the determination of flecainide in human plasma and its clinical application. Biomed Chromatogr. doi: 10.1002/bmc.3437 Google Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Matthew H. Slawson
    • 1
    • 2
    Email author
  • Kamisha L. Johnson-Davis
    • 3
  1. 1.ARUP Institute for Clinical and Experimental PathologySalt Lake CityUSA
  2. 2.ARUP LaboratoriesSalt Lake CityUSA
  3. 3.Department of Pathology, University of Utah Health Sciences Center, School of MedicineARUP Institute for Clinical and Experimental PathologySalt Lake CityUSA

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