Quantitation of Flecainide, Mexiletine, Propafenone, and Amiodarone in Serum or Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

  • Matthew H. SlawsonEmail author
  • Kamisha L. Johnson-Davis
Part of the Methods in Molecular Biology book series (MIMB, volume 1383)


Flecainide, mexiletine, propafenone, and amiodarone are antiarrhythmic drugs that are used primarily in the treatment of cardiac arrhythmias. The monitoring of the use of these drugs has applications in therapeutic drug monitoring and overdose situations. LC-MS/MS is used to analyze plasma/serum extracts with loxapine as the internal standard to ensure accurate quantitation and control for any potential matrix effects. Positive ion electrospray is used to introduce the analytes into the mass spectrometer. Selected reaction monitoring of two product ions for each analyte allows for the calculation of ion ratios which ensures correct identification of each analyte, while a matrix matched calibration curve is used for quantitation.

Key words

Flecainide Mexiletine Propafenone Amiodarone Plasma Serum UPLC Mass spectrometry 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Matthew H. Slawson
    • 1
    • 2
    Email author
  • Kamisha L. Johnson-Davis
    • 3
  1. 1.ARUP Institute for Clinical and Experimental PathologySalt Lake CityUSA
  2. 2.ARUP LaboratoriesSalt Lake CityUSA
  3. 3.Department of Pathology, University of Utah Health Sciences Center, School of MedicineARUP Institute for Clinical and Experimental PathologySalt Lake CityUSA

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