Quantitation of Ethyl Glucuronide and Ethyl Sulfate in Urine Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

  • Matthew H. Slawson
  • Kamisha L. Johnson-DavisEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1383)


Ethyl glucuronide and ethyl sulfate are minor conjugated metabolites of ethanol that can be detected in urine for several days after last ingestion of ethanol. The monitoring of ethanol use has both clinical and forensic applications and a longer detection window afforded by monitoring these metabolites is obvious. LC-MS/MS is used to analyze diluted urine with deuterated analogs of each analyte as internal standards to ensure accurate quantitation and control for any potential matrix effects. High aqueous HPLC is used to chromatograph the metabolites. Negative ion electrospray is used to introduce the metabolites into the mass spectrometer. Selected reaction monitoring of two product ions for each analyte allows for the calculation of ion ratios which ensures correct identification of each metabolite, while a matrix-matched calibration curve is used for quantitation.

Key words

Ethyl glucuronide Ethyl sulfate Urine Mass spectrometry 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Matthew H. Slawson
    • 1
    • 3
  • Kamisha L. Johnson-Davis
    • 2
    Email author
  1. 1.ARUP Institute for Clinical and Experimental PathologySalt Lake CityUSA
  2. 2.Department of Pathology, University of Utah Health Sciences Center, School of MedicineARUP Institute for Clinical and Experimental PathologySalt Lake CityUSA
  3. 3.ARUP LaboratoriesSalt Lake CityUSA

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