Comprehensive Urine Drug Screen by Gas Chromatography/Mass Spectrometry (GC/MS)

  • Bheemraj Ramoo
  • Melissa Funke
  • Clint Frazee
  • Uttam GargEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1383)


Drug screening is an essential component of clinical toxicology laboratory service. Some laboratories use only automated chemistry analyzers for limited screening of drugs of abuse and few other drugs. Other laboratories use a combination of various techniques such as immunoassays, colorimetric tests, and mass spectrometry to provide more detailed comprehensive drug screening. Mass spectrometry, gas or liquid, can screen for hundreds of drugs and is often considered the gold standard for comprehensive drug screening. We describe an efficient and rapid gas chromatography/mass spectrometry (GC/MS) method for comprehensive drug screening in urine which utilizes a liquid–liquid extraction, sample concentration, and analysis by GC/MS.

Key words

Toxicology GC/MS Drug analysis Comprehensive drug screening Drug screening 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Bheemraj Ramoo
    • 1
  • Melissa Funke
    • 1
  • Clint Frazee
    • 1
  • Uttam Garg
    • 1
    Email author
  1. 1.Department of Pathology and Laboratory MedicineChildren’s Mercy Hospitals and ClinicsKansas CityUSA

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