Cetirizine Quantification by High-Performance Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
A multiple reaction monitoring (MRM), positive ion electrospray ionization, LC/MS/MS method is described for the quantification of cetirizine. The compound was isolated from human plasma by protein precipitation using acetonitrile. Cetirizine d4 was used as an internal standard. Chromatographic conditions were achieved using a C18 column and a combination of ammonium acetate, water, and methanol as the mobile phase. MRMs were: cetirizine, 389.26 → 165.16, 201.09; cetirizine d4, 393.09 → 165.15, 201.10. Calibration curves were constructed by plotting the peak area ratios of the calibrators’ target MRM transition area to labeled internal standard target MRM transition area versus concentration.
Key wordsCetirizine Antihistamine H1-receptor Allergic rhinitis and chronic urticaria
- 6.Morita MR, Berton D, Boldin R, Barros FA, Meurer EC, Amarante AR, Campos DR, Calafatti SA, Pereira R, Abib E Jr et al (2008) Determination of levocetirizine in human plasma by liquid chromatography-electrospray tandem mass spectrometry: application to a bioequivalence study. J Chromatogr B Analyt Technol Biomed Life Sci 862:132–139CrossRefPubMedGoogle Scholar
- 12.Kang SW, Jang HJ, Moore VS, Park JY, Kim KA, Youm JR, Han SB (2010) Enantioselective determination of cetirizine in human plasma by normal-phase liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 878:3351–3357CrossRefPubMedGoogle Scholar