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Cetirizine Quantification by High-Performance Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

  • Ada Munar
  • Clint Frazee
  • Bridgette Jones
  • Uttam GargEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1383)

Abstract

A multiple reaction monitoring (MRM), positive ion electrospray ionization, LC/MS/MS method is described for the quantification of cetirizine. The compound was isolated from human plasma by protein precipitation using acetonitrile. Cetirizine d4 was used as an internal standard. Chromatographic conditions were achieved using a C18 column and a combination of ammonium acetate, water, and methanol as the mobile phase. MRMs were: cetirizine, 389.26 → 165.16, 201.09; cetirizine d4, 393.09 → 165.15, 201.10. Calibration curves were constructed by plotting the peak area ratios of the calibrators’ target MRM transition area to labeled internal standard target MRM transition area versus concentration.

Key words

Cetirizine Antihistamine H1-receptor Allergic rhinitis and chronic urticaria 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Ada Munar
    • 1
  • Clint Frazee
    • 3
  • Bridgette Jones
    • 2
  • Uttam Garg
    • 3
    Email author
  1. 1.Department of Pathology and Laboratory Medicine, Children’s Mercy Hospitals and ClinicsUniversity of Missouri School of MedicineKansas CityUSA
  2. 2.Department of PediatricsChildren’s Mercy Hospitals and ClinicsKansas CityUSA
  3. 3.Department of Pathology and Laboratory MedicineChildren’s Mercy Hospitals and ClinicsKansas CityUSA

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