Quantitation of Carisoprodol and Meprobamate in Urine and Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

  • Matthew H. Slawson
  • Kamisha L. Johnson-DavisEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1383)


Carisoprodol and meprobamate are centrally acting muscle relaxant/anxiolytic drugs that can exist in a parent–metabolite relationship (carisoprodol → meprobamate) or as a separate pharmaceutical preparation (meprobamate aka Equanil, others). The monitoring of the use of these drugs has both clinical and forensic applications in pain management applications and in overdose situations. LC-MS/MS is used to analyze urine or plasma/serum extracts with deuterated analogs of each analyte as internal standards to ensure accurate quantitation and control for any potential matrix effects. Positive ion electrospray is used to introduce the analytes into the mass spectrometer. Selected reaction monitoring of two product ions for each analyte allows for the calculation of ion ratios which ensures correct identification of each analyte, while a matrix-matched calibration curve is used for quantitation.

Key words

Carisoprodol Meprobamate Muscle relaxant Mass spectrometry 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Matthew H. Slawson
    • 2
    • 3
  • Kamisha L. Johnson-Davis
    • 1
    Email author
  1. 1.Department of PathologyUniversity of Utah Health Sciences Center, School of Medicine, ARUP Institute for Clinical and Experimental PathologySalt Lake CityUSA
  2. 2.ARUP Institute for Clinical and Experimental PathologySalt Lake CityUSA
  3. 3.ARUP LaboratoriesSalt Lake CityUSA

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