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Quantitative DNA Methylation Profiling in Cancer

  • Ole AmmerpohlEmail author
  • Andrea Haake
  • Julia Kolarova
  • Reiner Siebert
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1381)

Abstract

Epigenetic mechanisms including DNA methylation are fundamental for the regulation of gene expression. Epigenetic alterations can lead to the development and the evolution of malignant tumors as well as the emergence of phenotypically different cancer cells or metastasis from one single tumor cell. Here we describe bisulfite pyrosequencing, a technology to perform quantitative DNA methylation analyses, to detect aberrant DNA methylation in malignant tumors.

Key words

DNAmethylation profiling Epigenetics Bisulfite pyrosequencing 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Ole Ammerpohl
    • 1
    Email author
  • Andrea Haake
    • 1
  • Julia Kolarova
    • 1
  • Reiner Siebert
    • 1
  1. 1.Institute of Human GeneticsUniversity of Kiel and University Hospital Schleswig-Holstein, Campus KielKielGermany

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