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Cancer Gene Profiling pp 67–73Cite as

Gene Expression Analysis in the Age of Mass Sequencing: An Introduction

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1381))

Abstract

During the last years the technology used for gene expression analysis has changed dramatically. The old mainstay, DNA microarray, has served its due course and will soon be replaced by next-generation sequencing (NGS), the Swiss army knife of modern high-throughput nucleic acid-based analysis. Therefore preparation technologies have to adapt to suit the emerging NGS technology platform. Moreover, interpretation of the results is still time consuming and employs the use of high-end computers usually not found in molecular biology laboratories. Alternatively, cloud computing might solve this problem. Nevertheless, these new challenges have to be embraced for gene expression analysis in general.

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Author information

Authors and Affiliations

  1. Department of Surgery, TU Dresden, Fetscherstr. 74, Dresden, 01307, Germany

    Christian Pilarsky Ph.D. & Lahiri Kanth Nanduri

  2. Biotechnology Center, Technische Universität Dresden, Tatzberg 47-49, Dresden, 01307, Germany

    Janine Roy

Authors
  1. Christian Pilarsky Ph.D.
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  2. Lahiri Kanth Nanduri
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  3. Janine Roy
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Corresponding author

Correspondence to Christian Pilarsky Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Surgery, Universitätsklinikum Erlangen, Erlangen, Germany

    Robert Grützmann

  2. Department of Surgery, TU-Dresden, Dresden, Germany

    Christian Pilarsky

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Pilarsky, C., Nanduri, L.K., Roy, J. (2016). Gene Expression Analysis in the Age of Mass Sequencing: An Introduction. In: Grützmann, R., Pilarsky, C. (eds) Cancer Gene Profiling. Methods in Molecular Biology, vol 1381. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3204-7_4

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  • DOI: https://doi.org/10.1007/978-1-4939-3204-7_4

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3203-0

  • Online ISBN: 978-1-4939-3204-7

  • eBook Packages: Springer Protocols

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