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Tissue Microarrays

  • Ana-Maria Dancau
  • Ronald Simon
  • Martina Mirlacher
  • Guido SauterEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1381)

Abstract

Modern next-generation sequencing and microarray technologies allow for the simultaneous analysis of all human genes on the DNA, RNA, miRNA, and methylation RNA level. Studies using such techniques have lead to the identification of hundreds of genes with a potential role in cancer or other diseases. The validation of all of these candidate genes requires in situ analysis of high numbers of clinical tissues samples. The tissue microarray technology greatly facilitates such analysis. In this method minute tissue samples (typically 0.6 mm in diameter) from up to 1000 different tissues can be analyzed on one microscope glass slide. All in situ methods suitable for histological studies can be applied to TMAs without major changes of protocols, including immunohistochemistry, fluorescence in situ hybridization, or RNA in situ hybridization. Because all tissues are analyzed simultaneously with the same batch of reagents, TMA studies provide an unprecedented degree of standardization, speed, and cost efficiency.

Key words

TMA Tissue microarrays High-throughput in situ analysis IHC Immunohistochemistry FISH Fluorescence in situ hybridization Translational research 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Ana-Maria Dancau
    • 1
  • Ronald Simon
    • 1
  • Martina Mirlacher
    • 1
  • Guido Sauter
    • 1
    Email author
  1. 1.Institute of PathologyUniversity Medical Center Hamburg-EppendorfHamburgGermany

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