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Transcriptome Sequencing for the Detection of Chimeric Transcripts

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Cancer Gene Profiling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1381))

Abstract

The occurrence of chimeric transcripts has been reported in many cancer cells and seen as potential biomarkers and therapeutic targets. Modern high-throughput sequencing technologies offer a way to investigate individual chimeric transcripts and the systematic information of associated gene expressions about underlying genome structural variations and genomic interactions. The detection methods of finding chimeric transcripts from massive amount of short read sequence data are discussed here. Both assembly-based and alignment-based methods are used for the investigation of chimeric transcripts.

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Author information

Authors and Affiliations

  1. Department of Computer Science and Information Engineering, Asia University, No. 500, Liufeng Road, Wufeng District, Taichung City, 413, Taiwan

    Hsueh-Ting Chu

  2. Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan

    Hsueh-Ting Chu

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  1. Hsueh-Ting Chu
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Correspondence to Hsueh-Ting Chu .

Editor information

Editors and Affiliations

  1. Department of Surgery, Universitätsklinikum Erlangen, Erlangen, Germany

    Robert Grützmann

  2. Department of Surgery, TU-Dresden, Dresden, Germany

    Christian Pilarsky

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Chu, HT. (2016). Transcriptome Sequencing for the Detection of Chimeric Transcripts. In: Grützmann, R., Pilarsky, C. (eds) Cancer Gene Profiling. Methods in Molecular Biology, vol 1381. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3204-7_14

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  • DOI: https://doi.org/10.1007/978-1-4939-3204-7_14

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3203-0

  • Online ISBN: 978-1-4939-3204-7

  • eBook Packages: Springer Protocols

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