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Cancer Chemoprevention pp 13–19Cite as

Use of Buffy Coat miRNA Profiling for Breast Cancer Prediction in Healthy Women

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1379))

Abstract

MicroRNAs are key regulators of different biological processes and their deregulation is associated with the occurrence of many diseases among which cancer. Due to the higher stability of microRNAs and to the easiness in their detection both in organs than in biological fluids, many studies are turned toward potential use of this small molecules as biomarkers for the prediction and diagnosis of different types of cancer. Here we describe the experiment protocol that we have used for microRNA profiling analysis in buffy coat samples of women who developed breast cancer versus women who remained healthy during a 20 year follow-up period, with the aim to identify predictive microRNAs of breast cancer occurrence.

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Acknowledgment

We are indebted to the 10,786 ORDET participants. We also thank Dr. Paolo Contiero and the staff of the Lombardy Cancer Registry for technical assistance. This work was supported by Department of Defense grant W81 XWH 04 1 0195 and by the Veronesi Foundation.

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Authors and Affiliations

  1. Translational Oncogenomics Unit, Regina Elena Italian National Cancer Institute, Rome, Italy

    Sara Donzelli

  2. Laboratory of Translational Oncogenomics, Molecular Medicine Department, Regina Elena National Cancer Institute, Rome, Italy

    Giovanni Blandino

  3. Department of Oncology, Faculty of Health Science, McMaster University, 1280 Main Street West, Hamilton, ON, Canada

    Paola Muti

Authors
  1. Sara Donzelli
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  2. Giovanni Blandino
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  3. Paola Muti
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Corresponding author

Correspondence to Paola Muti .

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Editors and Affiliations

  1. Molecular Cancer Chemoprevention Unit, Molecular Medicine Department; Department of Oncology, Juravinski Cancer Center-McMaster, Regina Elena National Cancer Institute; University Hamilton, Rome; Ontario, Italy

    Sabrina Strano

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Donzelli, S., Blandino, G., Muti, P. (2016). Use of Buffy Coat miRNA Profiling for Breast Cancer Prediction in Healthy Women. In: Strano, S. (eds) Cancer Chemoprevention. Methods in Molecular Biology, vol 1379. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3191-0_2

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  • DOI: https://doi.org/10.1007/978-1-4939-3191-0_2

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3190-3

  • Online ISBN: 978-1-4939-3191-0

  • eBook Packages: Springer Protocols

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