Abstract
RT-PCR is an invaluable tool for the detection and characterization of mRNA. Cancer cell lines are treated with crude plant extracts and RNA is extracted and purified with DNase prior to RT-PCR. RT-PCR first-strand cDNA synthesis is done using random primers and can be refrigerated at 4 °C. PCR from the stored cDNA is performed using transcript-specific primers and electrophoresed on a molecular grade agarose gel to separate the splice variants.
An erratum to this chapter is available at http://dx.doi.org/10.1007/978-1-4939-3191-0_19
An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-1-4939-3191-0_19
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Acknowledgement
The British Royal Society, the South African Medical Research Council, the Oppenheimer Trust, and the National Research Foundation of South Africa supported this work.
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Dlamini, Z., Mbita, Z., Bates, D. (2016). South African Herbal Extracts as Potential Chemopreventive Agents: Screening for Anticancer Splicing Activity. In: Strano, S. (eds) Cancer Chemoprevention. Methods in Molecular Biology, vol 1379. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3191-0_18
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DOI: https://doi.org/10.1007/978-1-4939-3191-0_18
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