Abstract
Chemoresistance is a major challenge for cancer therapy and drives tumor relapse. The emergence, within the treated tumor mass, of specific cancer cell subpopulations endowed with high tolerance to the microenvironment stress induced by therapy is being growingly recognized as a mechanism of tumor progression. To obtain detailed information with regard to the pathways underlying survival, expansion, and microenvironmental cross talk of such chemoresistant cell subpopulations may be instrumental for cancer chemoprevention. Additionally, the obtained cell subpopulations may be used for direct screening of cancer chemopreventive compounds, in appropriate experimental settings. Here we report detailed experimental procedures that we and others have setup in order to obtain cell cultures enriched for chemoresistant cells from both malignant pleural mesothelioma specimens and primary cell cultures. We provide indications for the purification and characterization of those chemoresistant cell populations and to generally validate the obtained enriched cell populations for their chemoresistance.
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References
Abdullah LN, Chow EK (2013) Mechanisms of chemoresistance in cancer stem cells. Clin Transl Med 2(1):3
Cojoc M, Mabert K, Muders MH et al (2015) A role for cancer stem cells in therapy resistance: Cellular and molecular mechanisms. Semin Cancer Biol 31:16–27
Vidal SJ, Rodriguez-Bravo V, Galsky M et al (2014) Targeting cancer stem cells to suppress acquired chemotherapy resistance. Oncogene 33(36):4451–4463
Canino C, Mori F, Cambria A et al (2012) SASP mediates chemoresistance and tumor-initiating-activity of mesothelioma cells. Oncogene 31(26):3148–3163
Cioce M, Canino C, Goparaju C et al (2014) Autocrine CSF-1R signaling drives mesothelioma chemoresistance via AKT activation. Cell Death Dis 5:e1167
Cioce M, Gherardi S, Viglietto G et al (2010) Mammosphere-forming cells from breast cancer cell lines as a tool for the identification of CSC-like- and early progenitor-targeting drugs. Cell Cycle 9(14):2878–2887
Lanfrancone L et al (1992) Human peritoneal mesothelial cells produce many cytokines (granulocyte colony-stimulating factor [CSF], granulocyte-monocyte-CSF, macrophage-CSF, interleukin-1 [IL-1], and IL-6) and are activated and stimulated to grow by IL-1. Blood 80(11):2835–2842
Cortes-Dericks L, Froment L, Boesch R et al (2014) Cisplatin-resistant cells in malignant pleural mesothelioma cell lines show ALDH(high)CD44(+) phenotype and sphere-forming capacity. BMC Cancer 14:304
Luo Y, Dallaglio K, Chen Y et al (2012) ALDH1A isozymes are markers of human melanoma stem cells and potential therapeutic targets. Stem Cells 30(10):2100–2113
Acknowledgement
M.C. was supported by the NYU Cancer Institute Cancer Center Support Grant’s Developmental Project Program (P30CA016087).
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Canino, C., Cioce, M. (2016). Isolation of Chemoresistant Cell Subpopulations. In: Strano, S. (eds) Cancer Chemoprevention. Methods in Molecular Biology, vol 1379. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3191-0_13
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DOI: https://doi.org/10.1007/978-1-4939-3191-0_13
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3190-3
Online ISBN: 978-1-4939-3191-0
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