Abstract
The well recognized “bottleneck” in sample preparation of the difficult samples is efficient lysis, homogenization, and grinding of the sample. Herein we describe the principles of operations and applications of the FastPrep® family of systems and kits for lysing, homogenization, and grinding, utilizing the modified bead beating technique based on the combination of simultaneous cascade impaction, mechanical shearing and hydrodynamic/vortex shearing. The chapter provides an overview of the systems, lysing matrices and application examples relevant to efficient lysis, grinding, and homogenization for downstream molecular biology, proteomics, or general biochemistry sample prep workflow for animals, plants, microbes, and environmental samples which are difficult to lyse.
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References
Micic M, Whyte JD (2012) Breaking the sample-prep bottleneck: automated lysis systems streamline workflows. GEN 32(2):31–33. doi:10.1089/gen.32.2.16
Susanne R, Berglund CW, Schwietert AA, Jones RL, Stern JL, Goldberg Z (2007) Optimized methodology for sequential extraction of RNA and protein from small human skin biopsies. J Invest Dermatol 127:349–353. doi:10.1038/sj.jid.5700557
Jiang J, Alderisio KA, Singh A, Xiao L (2005) Development of procedures for direct extraction of cryptosporidium DNA from water concentrates and for relief of PCR inhibitors. Appl Environ Microbiol 71(3):1135–1141. doi:10.1128/AEM.71.3.1135-1141.2005
Hudson KD, Corfe BM, Helen Kemp E, Feavers IM, Coote PJ, Moir A (2001) Localization of GerAA and GerAC Germination Proteins in the Bacillus subtilis Spore. J Bacteriol 183(14):4317–4322. doi:10.1128/JB.183.14.4317-4322.2001
Bart JF, Keijser ATB, Rauwerda H, Schuren F, Montijn R, van der Spek H, Brul S (2007) Analysis of temporal gene expression during bacillus subtilis spore germination and outgrowth. J Bacteriol 189(9):3624–3634. doi:10.1128/JB.01736-06
Cheun HI, Makino SI, Watarai M, Erdenebaatar J, Kawamoto K, Uchida I (2003) Rapid and effective detection of anthrax spores in soil by PCR. J Appl Microbiol 95(4):728–733. doi:10.1046/j.1365-2672.2003.02038.x
Roesti D, Ineichen K, Braissant O, Redecker D, Wiemken A, Aragno M (2005) Bacteria associated with spores of the arbuscular mycorrhizal fungi glomus geosporum and glomus constrictum. Appl Environ Microbiol 71(11):6673–6679. doi:10.1128/AEM.71.11.6673-6679.2005
Mincer TJ, Fenical W, Jensen PR (2005) Culture-dependent and culture-independent diversity within the obligate marine actinomycete genus salinispora. Appl Environ Microbiol 71(11):7019–7028. doi:10.1128/AEM.71.11.7019-7028.2005
Acknowledgement
The authors would like to acknowledge contributions of several key individuals in developing, launching, and maintaining the FastPrep systems, instrumentation, and kits line of products. We would like especially to acknowledge Hon. Mr. Milan Panic, president and CEO of MP Biomedicals for his continuous support of the development of product line, Ms. Elisabeth Kylin, Dr. David Simpson (both currently with Sunrise Science Products), Dr. Daniel Weissbart, Mr. Vidhjut Jani, Mr. Nebojsa Pesic, and late Mr. Fred Andrea from MP Biomedicals, Dr. Horacio Kido and Mr James Northon from RotaPrep Inc, Mr. Satyendra and Ms. Nisha Johari, Mr. Tejas Shah, and Ms. Reena Daga from Johari Digitial Healthcare PTY Ltd, for their respective contributions to advancement of the bead beating sample prep technologies and FastPrep products line. We would also like to acknowledge Ms. Amy Stagl, for her help in preparation of figures and editing of the manuscript.
The work on development of te FastPrep-24-5G latest generation instrument was supported in part by the European Union’s Seventh Framework Programme, HighGlycan (contract No. 278535).
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Mićić, M., Whyte, J.D., Karsten, V. (2016). High Performance Bead Beating Based Lysing, Homogenization and Grinding for DNA, RNA and Proteins Extraction with FastPrep® Systems. In: Micic, M. (eds) Sample Preparation Techniques for Soil, Plant, and Animal Samples. Springer Protocols Handbooks. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3185-9_8
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DOI: https://doi.org/10.1007/978-1-4939-3185-9_8
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3184-2
Online ISBN: 978-1-4939-3185-9
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