Abstract
The preparation of high quality and quantity RNA is desirable for several different applications. The mini-prep protocol described here can be used to obtain high purity, total RNA from the seeds of several plant species, including Helianthus tuberosus L., Helianthus annuus L., Arachis hypogaea L., Zea mays L., Coix lacryma-jobi L., Sesamum indicum L., Phaseolus vulgaris L., and Mucuna pruriens DC. The extracted RNA was compatible for further analyses such as qPCR for RNA and gene expression quantification.
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Acknowledgements
This work was financially supported by the Higher Education Research Promotion and the National Research University Project of Thailand, Office of the Higher Education Commission, through the Food and Functional Food Research Cluster of Khon Kaen University; the research funding from Khon Kaen University to the corresponding author; and by the Thailand Research Fund (TRF), the commission for Higher Education (CHE), and Khon Kaen University (KKU) through the Distinguish Research Professor Grant to Aran Patanothai.
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Wangsomnuk, P.P., Ruttawat, B., Rittithum, W., Wangsomnuk, P., Jogloy, S., Patanothai, A. (2016). RNA Extractions from Difficult to Prepare and High Starch Content Seeds. In: Micic, M. (eds) Sample Preparation Techniques for Soil, Plant, and Animal Samples. Springer Protocols Handbooks. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3185-9_19
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DOI: https://doi.org/10.1007/978-1-4939-3185-9_19
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3184-2
Online ISBN: 978-1-4939-3185-9
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