Abstract
The Na,K-ATPase is a plasma membrane enzyme that catalyzes active ion transport by the hydrolysis of ATP. Its activity in vivo is determined by many factors, particularly the concentration of intracellular sodium ions. It is the target of the cardiac glycoside class of drugs and of endogenous regulators. Its assay is often an endpoint in the investigation of physiological processes, and it is a promising drug target. As described in this unit, its enzymatic activity can be determined in extracts from tissues by test tube assay using a spectrophotometer or 32P-ATP. The protocols in this chapter measure inorganic phosphate as the end product of hydrolysis of ATP.
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Acknowledgement
This work was supported by NIH grants HL036271, NS045283, EY014390, NS050696, and NS081558. The author is grateful to all of the laboratory members who mastered and refined this methodology.
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Sweadner, K.J. (2016). Colorimetric Assays of Na,K-ATPase. In: Bublitz, M. (eds) P-Type ATPases. Methods in Molecular Biology, vol 1377. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3179-8_10
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DOI: https://doi.org/10.1007/978-1-4939-3179-8_10
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3178-1
Online ISBN: 978-1-4939-3179-8
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