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Luciferase Reporter Assays to Assess Liver X Receptor Transcriptional Activity

  • Matthew C. Gage
  • Benoit Pourcet
  • Inés Pineda-TorraEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1376)

Abstract

Luciferase reporter assays are sensitive and accurate tests that enable the analysis of regulatory sequences, the magnitude of transcriptional activity by transcription factors, and the discovery of gene regulatory elements and small-molecule modulators with high levels of precision. This is made possible through detection of bioluminescence produced by luciferase-coding reporters in a wide range of cellular environments. These assays are routinely used to analyze the activity of transcription factors, including the lipid-activated liver X receptor (LXR), in response to different stimuli as well as for the identification of their ligands. In this chapter we describe in detail the assays performed to investigate LXR activity in a macrophage-like cell line (RAW 267.4). These can be easily adapted to other nuclear receptors and transcription factors.

Key words

Nuclear receptors Liver X receptor Luciferase Reporter assay Luminescence Macrophage RAW 267.4 

Notes

Acknowledgement

The authors would like to thank the support of the Medical Research Council (New Investigator Grant G0801278), International Reintegration Program (Grant PIRG02-GA-2007-224867) and British Heart Foundation (PG/13/10/30000) to IPT.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Matthew C. Gage
    • 1
  • Benoit Pourcet
    • 1
    • 2
  • Inés Pineda-Torra
    • 1
    Email author
  1. 1.Division of Medicine, Centre for Clinical PharmacologyUniversity College of LondonLondonUK
  2. 2.UMR INSERM 1011, Institut Pasteur de LilleUniversité Lille Nord de FranceLille CedexFrance

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