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Assay for CDP-Diacylglycerol Generation by CDS in Membrane Fractions

  • Mark G. WaughEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1376)

Abstract

CDP-DAG is a liponucleotide formed by the condensation of CTP with the phospholipid phosphatidic acid in a reaction catalyzed by CDP-DAG synthase (CDS). CDP-DAG is required for the synthesis of phosphatidylinositol; the parent molecule whence all seven phosphoinositides including the signaling molecules PI4P, PI(4,5)P2, and PI(3,4,5)P3 are derived. This protocol describes a highly sensitive radiometric assay to detect the generation of CDP-DAG on isolated biological membrane fractions.

Key words

CDP-DAG CDS Liponucleotide Assay Endoplasmic reticulum Membrane 

Notes

Acknowledgements

MGW acknowledges financial support from the Royal Free Charity.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.School of Life and Medical SciencesUniversity College LondonLondonUK

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