Assay for CDP-Diacylglycerol Generation by CDS in Membrane Fractions
CDP-DAG is a liponucleotide formed by the condensation of CTP with the phospholipid phosphatidic acid in a reaction catalyzed by CDP-DAG synthase (CDS). CDP-DAG is required for the synthesis of phosphatidylinositol; the parent molecule whence all seven phosphoinositides including the signaling molecules PI4P, PI(4,5)P2, and PI(3,4,5)P3 are derived. This protocol describes a highly sensitive radiometric assay to detect the generation of CDP-DAG on isolated biological membrane fractions.
Key wordsCDP-DAG CDS Liponucleotide Assay Endoplasmic reticulum Membrane
MGW acknowledges financial support from the Royal Free Charity.
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