Abstract
Lipid rafts have been drawing extensive attention as a signaling platform. To investigate molecular interactions in lipid rafts, we often need to observe molecules in the plasma membranes of living cells because chemical fixation and subsequent immunostaining with divalent or multivalent antibodies may change the location of the target molecules. In this chapter, we describe how to examine dynamics of raft-associated glycosylphosphatidylinositol (GPI)-anchored receptors and interactions of the receptors with downstream signaling molecules by single-particle tracking or single-molecule imaging techniques.
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Acknowledgement
This work was supported in part by Grants-in-Aid for Specific Research (B) (No. 24370055) and by Innovative Areas (No. 2311002) from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
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Suzuki, K.G.N. (2016). Single-Molecule Imaging of Signal Transduction via GPI-Anchored Receptors. In: Waugh, M. (eds) Lipid Signaling Protocols. Methods in Molecular Biology, vol 1376. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3170-5_19
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DOI: https://doi.org/10.1007/978-1-4939-3170-5_19
Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-3170-5
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