Determination and Characterization of Tetraspanin-Associated Phosphoinositide-4 Kinases in Primary and Neoplastic Liver Cells

  • Krista RomboutsEmail author
  • Vinicio CarloniEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1376)


Accumulating evidence implicates phosphoinositide 4-phosphate as a regulatory molecule in its own right recruiting specific effector proteins to cellular membranes. Here, we describe biochemical and immunocytochemical methods to evaluate tetraspanin-associated phosphoinositide-4 kinases activity in primary human hepatic stellate cells (hHSC) and neoplastic hepatoblastoma cells.

Key words

Thin layer chromatography Tetraspanins Tetraspanin-enriched microdomains Human hepatic stellate cells Density gradient centrifugation Hepatocellular carcinoma cells 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Division of Medicine, Institute for Liver & Digestive HealthRoyal Free Hospital, University College London (UCL)LondonUK
  2. 2.Department of Experimental and Clinical Medicine, Center for Research, Transfer and High Education, DENOtheUniversity of FlorenceFlorenceItaly

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