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Detection of Isolated Mitochondria-Associated ER Membranes Using the Sigma-1 Receptor

  • Abasha Lewis
  • Shang-Yi Tsai
  • Tsung-Ping SuEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1376)

Abstract

The interface between the endoplasmic reticulum (ER) and mitochondria referred to as the MAM (mitochondria-associated ER membrane) plays important roles in many physiological functions. A specific marker for this important entity of cellular structure is urgently needed. Thus, we propose in this method chapter that the membrane-bound ER chaperone sigma-1 receptor serves as an ideal marker for the MAM. We describe in detail the preparation and purification of the MAM by using the sigma-1 receptor as the marker and demonstrate the uniqueness of this marker by using a variety of cells, peripheral and neuronal.

Key words

Sigma-1 receptor Chaperone Endoplasmic reticulum Mitochondria MAM SDS/PAGE electrophoresis Fractionation 

Notes

Acknowledgment

This work is supported by the Intramural Research Program of the National Institute on Drug Abuse, National Institutes of Health, of the Department of Health and Human Services of the United States of America.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Cellular Pathobiology SectionIRP, DHHS, NIDA, NIHBaltimoreUSA

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