Abstract
Small interfering RNA (siRNA) is routinely used as a biological tool to silence specific genes, and is under active investigation in cancer treatment strategies. Noninvasive magnetic resonance spectroscopy (MRS) provides the ability to assess the functional effects of siRNA-mediated gene silencing in cultured cancer cells, and following nanoparticle-based delivery in tumors in vivo. Here we describe the use of siRNA to downregulate choline kinase, a critical enzyme in choline phospholipid metabolism of cancer cells and tumors, and the use of 1H MRS of cells and 1H magnetic resonance spectroscopic imaging (MRSI) of tumors to assess the efficacy of the downregulation.
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Acknowledgements
Support from P50 CA103175, R01 CA82337, R01 CA73850, R01 CA136576, and R01 CA138515 is gratefully acknowledged.
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Penet, MF., Chen, Z., Mori, N., Krishnamachary, B., Bhujwalla, Z.M. (2016). Magnetic Resonance Spectroscopy of siRNA-Based Cancer Therapy. In: Medarova, Z. (eds) RNA Imaging. Methods in Molecular Biology, vol 1372. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3148-4_3
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DOI: https://doi.org/10.1007/978-1-4939-3148-4_3
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3147-7
Online ISBN: 978-1-4939-3148-4
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