Abstract
Microvesicles and exosomes are released to the extracellular milieu and are detectable in body fluids. They act as unique vehicles for cargo transfer to other cells/tissues. They contain a set of membrane receptors, intracellular proteins and nucleic acids. Here, we describe basic techniques for their isolation and characterization from cell culture media or body fluids. We also describe critical techniques for characterizing their cargo (miRNAs) and their protein content. Finally, we present labeling methods for their use in in vitro procedures and for their in vivo delivery and subsequent analysis of their cargo transfer to multiple cell types.
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Acknowledgment
This work was supported by the Spanish Ministry of Economy and Competitiveness SAF2012-40056 to F.L, “UTE project FIMA” agreement, the Cancer Research Thematic Network of the Health Institute Carlos III (RTICC RD12/0036/0066), European Regional Development Fund (ERDF) “Una manera de hacer Europa.”
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Valencia, K., Lecanda, F. (2016). Microvesicles: Isolation, Characterization for In Vitro and In Vivo Procedures. In: Medarova, Z. (eds) RNA Imaging. Methods in Molecular Biology, vol 1372. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3148-4_14
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DOI: https://doi.org/10.1007/978-1-4939-3148-4_14
Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-3148-4
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