Abstract
Unveiling the function of a novel protein is a challenging task that requires careful experimental design. Yeast cytokinesis is a conserved process that involves modular structural and regulatory proteins. For such proteins, an important step is to identify their domains and structural organization. Here we briefly discuss a collection of methods commonly used for sequence alignment and prediction of protein structure that represent powerful tools for the identification homologous domains and design of structure-function approaches to test experimentally the function of multi-domain proteins such as those implicated in yeast cytokinesis.
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Acknowledgements
We would like to thank Carlos Vazquez de Aldana for very helpful discussions. A.P. is supported by Agence Nationale de la Recherche, Ligue contre le cancer Comité de Paris and Fondation ARC pour la Recherche. A.P. is a member of Labex CelTisPhyBio, which is part of Idex PSL*. S.A.R. received postdoctoral fellowships from Fundacion Ramon Areces and Marie Curie FP7 program.
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Rincon, S.A., Paoletti, A. (2016). From Structure to Function: A Comprehensive Compendium of Tools to Unveil Protein Domains and Understand Their Role in Cytokinesis. In: Sanchez-Diaz, A., Perez, P. (eds) Yeast Cytokinesis. Methods in Molecular Biology, vol 1369. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3145-3_24
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DOI: https://doi.org/10.1007/978-1-4939-3145-3_24
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