Abstract
Multiple membrane-binding proteins are key players in cytokinesis in yeast and other organisms. In vivo techniques for analyzing protein–membrane interactions are currently limited. In vitro assays allow characterization of the biochemical properties of these proteins to build a mechanistic understanding of protein–membrane interactions during cytokinesis. Here, we describe two in vitro assays to characterize FCH-Bin/Amphyphysin/RVS (F-BAR) domains and other protein’s interactions with membranes: liposome co-pelleting and giant unilamellar vesicle fluorescent binding.
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Acknowledgements
This work was supported by NIH grant GM101035 to K.L.G and AHA fellowship 15PRE21780003 to N.A.M.
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McDonald, N.A., Gould, K.L. (2016). Characterization of Cytokinetic F-BARs and Other Membrane-Binding Proteins. In: Sanchez-Diaz, A., Perez, P. (eds) Yeast Cytokinesis. Methods in Molecular Biology, vol 1369. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3145-3_13
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DOI: https://doi.org/10.1007/978-1-4939-3145-3_13
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3144-6
Online ISBN: 978-1-4939-3145-3
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