Abstract
Fluorescence in situ hybridization (FISH) has become one of the most important technologies applied in plant molecular cytogenetic research. FISH technique has been not only well applied in physical mapping and genomic studies, but also served as an indispensable tool in tracing the individual chromosome during cell division. This chapter provides protocols for basic FISH analysis using rice as a model, which can also be adapted to other model plant species.
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References
Jiang J, Gill BS (2006) Current status and the future of fluorescence in situ hybridization (FISH) in plant genome research. Genome 49:1057–1068
Langer-Safer PR, Levine M, Ward DC (1982) Immunological method for mapping genes on Drosophila polytene chromosomes. Proc Natl Acad Sci U S A 79:4381–4385
Goff SA (1999) Rice as a model for cereal genomics. Curr Opin Plant Biol 2:86–89
Cheng Z, Stupar RM, Gu M, Jiang J (2001) A tandemly repeated DNA sequence is associated with both knob-like heterochromatin and a highly decondensed structure in the meiotic pachytene chromosomes of rice. Chromosoma 110:24–31
Cheng Z, Dong F, Langdon T, Ouyang S, Buell CR, Gu M, Blattner FR, Jiang J (2002) Functional rice centromeres are marked by a satellite repeat and a centromere-specific retrotransposon. Plant Cell Online 14:1691
Cheng Z, Buell CR, Wing RA, Gu M, Jiang J (2001) Toward a cytological characterization of the rice genome. Genome Res 11:2133–2141
Tang X, Bao W, Zhang W, Cheng Z (2007) Identification of chromosomes from multiple rice genomes using a universal molecular cytogenetic marker system. J Integr Plant Biol 49:953–960
Acknowledgement
This work was supported by grants from the National Natural Science Foundation of China (31401357).
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Li, Y., Cheng, Z. (2016). Fluorescence In Situ Hybridization on Rice Chromosomes. In: Caillaud, MC. (eds) Plant Cell Division. Methods in Molecular Biology, vol 1370. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3142-2_8
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DOI: https://doi.org/10.1007/978-1-4939-3142-2_8
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3141-5
Online ISBN: 978-1-4939-3142-2
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